An introduction to the analysis of tetrahydrocannabinol thc

The competitive binding takes place between the antibody, the THC in the sample, and a constant concentration of tracer added to the sample.

An introduction to the analysis of tetrahydrocannabinol thc

Of these 12, cases, there were 4, cannabinoid screens CS requested. After this normalization, there were 1, samples that were confirmed positive for THC and included in the analysis. The mean and median THC concentrations were 8. The data presented illustrate a statistically significant increase in CS that result in positive THC confirmations.

Introduction Marijuana is the most commonly used illicit drug in the USA 1. Between andthe rate of marijuana use increased from 5. Both the use of marijuana and public support for its legalization have recently undergone significant increases in the USA.

This attitude is reflected in the recent legalization of recreational marijuana in the states of Colorado and Washington. These unprecedented policy changes spur awareness of the potential for individuals to drive under the influence of marijuana.

Smoking is the most common route of marijuana administration as it provides rapid delivery of THC to the brain, quickly producing the desired effects of the drug. Peak effects of smoking are generally noted 20—30 min after use, with blood concentrations decreasing to low levels after 3 h and to baseline after 4 h in most users 34.

An introduction to the analysis of tetrahydrocannabinol thc

Based on the pharmacokinetic profile of THC, in particular its rapid metabolization to detectable metabolites such as THC—COOH, detection of THC in blood is essential to determine whether a subject is under the influence of, and potentially impaired by, marijuana.

This underscores the imperative nature of timely collection of blood samples during driving under the influence of drugs DUID investigations.

THC is known to impair driving 6— THC impairs cognition, psychomotor function, and driving performance in a dose-related manner. Highly automated driving tasks, such as road tracking control, are the most affected by THC impairment 67. THC serum concentrations are greater than those quantitated in whole blood.

The state of Colorado is currently under scrutiny as the subject of what is effectively an ongoing experiment in progressive marijuana policy.

Use of medical marijuana MMJ has been legal in the state of Colorado since As witnessed in a number of states, the Ogden memo has proved to be only a guideline, not a guarantee of nonprosecution at the federal level.

Ultrasonic Sample Preparation for THC Analysis

Currently, more thanapplications have been submitted, and there are overactive MMJ cardholders in Colorado On 12 Novemberthe passing of Amendment 64 legalized private consumption of cannabis in Colorado; it was ratified in December Most recently, on 1 Januaryrecreational marijuana retail stores opened to the public A reorganization of available toxicological testing laboratories in Colorado during this time period led to a large sample influx.

In order to normalize for this occurrence, the above-listed criteria were also analyzed for a single Denver metropolitan area LE agency for which ChemaTox performed exclusive testing throughout the entirety of the time frame examined.

Limited data from January and February of were available at the time of the study.

An introduction to the analysis of tetrahydrocannabinol thc

The results from January and February of all four years were analyzed in addition to the full year data to allow for better comparison. Limited information is submitted with the samples and includes, but is not limited to, agency name, date and time of the incident, date and time of the blood draw, offense, testing requested, medications taken, and chain of custody information.

The officer occasionally provides other case information, including limited demographic or narrative case information. Of these 12, cases, there were 4, CS requested.Introduction. Several methods have been described for monitoring prenatal exposure to marijuana through analysis of deltatetrahydrocannabinol (THC) and its metabolites in blood, urine, hair, placenta, amniotic fluid, gastric aspirate.

Introduction

 Tetrahydrocannabinol Tetrahydrocannabinol (THC) is commonly known and popularized by its significant presence in the cannabis plant, a.k.a., marijuana. Raphael Mechoulam and Yechiel Gaoni, two Israeli scientists working at the Weizmann Institute of Science, first isolated the organic drug in Introduction Several methods have been described for monitoring prenatal exposure to marijuana through analysis of deltatetrahydrocannabinol (THC) and its metabolites in blood, urine, hair, placenta, amniotic fluid, gastric aspirate (1), in umbilical tional marijuana use through analysis of meconium.

In the case of cannabis, this can be done by analyzing for the psychoactive substance, tetrahydrocannabinol (THC), and its major metabolite, carboxytetrahydrocannabinol (THCCOOH). THC can be analyzed by GC directly, but because THCCOOH contains an acid group in its structure it requires derivatization.

Medical Cannabis Series An Introduction to the Biochemistry and Pharmacology of Medical Cannabis List of References British Columbia Compassion Club Society.

Analysis of Δ9-tetrahydrocannabinol Driving Under the Influence of Drugs Cases in Colorado from January to February Sarah Urfer. Introduction. Marijuana is the Δ9-tetrahydrocannabinol (THC).

The method was validated according to protocols, which included assessment of accuracy, precision, robustness, stability in meconium and in-process stability, interference and sensitivity and specificity. The method uses deuterated internal standards for both analytes.
Of these 12, cases, there were 4, cannabinoid screens CS requested.
THC Analysis with Triple Quadrupole GC-MS Because the concentration of the metabolite in oral fluid is quite low, extremely sensitive analytical methods are necessary. The requisite sensitivity was achieved by a simple, rapid derivatization of the compound after extraction.
For the preparation of the samples, the dried plant material must be macerated and grinded to a homogeneous powder. For the ultrasonic extraction, mg of the cannabis plant powder and 5mL methanol-chloroform mixture ratio of 9:
Rapid screening tests for the detection of deltaTHC